A rapid and sensitive method for the quantitative determination of free amino groups during solid-phase peptide synthesis has been developed. The technique involves the reaction of the free amine with ninhydin under carefully controlled conditions and the determination of the resulting chromophore i
β¦ LIBER β¦
The monitoring of reactions in solid-phase peptide synthesis with picric acid
β Scribed by B.F. Gisin
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 152 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0003-2670
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## Abstract The dangerous liquid HF, which in addition causes many sideβreactions, has successfully been replaced by the easyβtoβhandle organic acid methanesulfonic acid for the cleavage of small peptides from the standard Merrifield resin.