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The localization of tRNA 2 Asp genes from Drosophila melanogaster by “in situ” hybridization

✍ Scribed by Schmidt, Thomas ;Egg, Albert H. ;Kubli, Eric

Publisher
Springer
Year
1978
Tongue
English
Weight
560 KB
Volume
164
Category
Article
ISSN
0026-8925

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✦ Synopsis

Transfer RNAAsp2delta was isolated from Drosophila melanogaster by affinity chromatography on concanavalin A-Sepharose. The tRNA was iodinated "in vitro" with Na [125I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAAsp2 to the left arm of the second chromosome in the regions 29 D and E.

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