Localization of theIGHG, PRKACB, andTNP2genes in pigs by in situ hybridization

✍ Scribed by F. Gu; B. P. Chowdhary; M. Johansson; L. Andersson; I. Gustavsson

• Publisher: Springer-Verlag
• Year: 1994
• Tongue: English
• Weight: 367 KB
• Volume: 5
• Category: Article
• ISSN: 0938-8990
• DOI: 10.1007/bf00360544

✦ Synopsis

The porcine genes encoding the immunoglobulin gamma heavy chain (IGHG), cAMP-dependent protein kinase catalytic beta subunit (PRKACB), and transition protein 2 (TNP2) were mapped to Chromosomes (Chrs) 7 q25-q26, 6q31-q33, and 3p13-cent, respectively, by in situ hybridization. Localization of the IGHG gene confirms the assignment of linkage group III to Chr 7. Our results show that the IGHG locus in pigs, similar to the situation in other mammalian species, viz. humans, mouse, cattle, and river buffaloes, is located on the terminal region of the chromosome. The assignment of the PRKACB gene extends the homology observed between porcine Chr 6q and human Chr 1p. Mapping of the TNP2 gene provides the first marker assigned to the p arm of Chr 3 in pigs. The present study contributes to the development of the physical gene map in pigs and also bears significance in terms of comparative gene mapping.