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Chromosomal localization of the human myeloperoxidase gene by in situ hybridization using oligonucleotide probes

✍ Scribed by Dr. Sharif R. Zaki; Garth E. Austin; Wing C. Chan; Ann L. Conaty; Suzanne Trusler; Sam Trappier; Rusha B. Lindsey; Dr. David C. Swan

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 382 KB
Volume: 2
Category: Article
ISSN: 1045-2257
DOI: 10.1002/gcc.2870020403

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✦ Synopsis

Abstract

Oligonucleotide probes have been used to map the myeloperoxidase (MPO) gene locus to chromosome bands 17q21–22. This is in agreement with results reported using conventional cDNA probes. No evidence for the existence of a second MPO gene locus was obtained. Six synthetic 72‐base oligonucleotides, corresponding to different exon regions of the MPO gene, were tritium‐labeled and used as in situ hybridization probes. Synthetic oligonucleotide probes offer a useful alternative to conventional DNA probes for gene mapping.

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