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The length polymorphism in the 5′ flanking region of the human β-globin gene with denaturing gradient gel electrophoresis in a Japanese population

✍ Scribed by Norio Takahashi; Keiko Hiyama; Mieko Kodaira; Chiyoko Satoh

Publisher
Springer
Year
1991
Tongue
English
Weight
266 KB
Volume
87
Category
Article
ISSN
0340-6717

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✦ Synopsis

The ATTTT repeat polymorphism located approximately 1,400 base pairs (bp) upstream from the beta-globin structural gene was analyzed by denaturing gradient gel electrophoresis (DGGE) of RNA:DNA duplexes. A study of 81 unrelated Japanese from Hiroshima revealed a sequence heteromorphism in this site. The alleles with five and six repeats of the ATTTT unit, which have been reported, were found in polymorphic proportions. Two unreported alleles were also detected, the first, in two persons, characterized by seven repeats and the other, in a single person, having an A-to-G nucleotide substitution in the fifth repeat.

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