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Detection by denaturing gradient gel electrophoresis of a new polymorphism in the apolipoprotein B gene

✍ Scribed by Maria Navajas; Anne-Marie Laurent; Jean-François Moreel; Ashraf Ragab; Jean-Pierre Cambou; Gérard Cuny; François Cambien; Gérard Roizès

Publisher
Springer
Year
1990
Tongue
English
Weight
247 KB
Volume
86
Category
Article
ISSN
0340-6717

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✦ Synopsis

The apolipoprotein B gene is subject to mutations that may be important in coronary heart diseases. We have used polymerase chain reaction and denaturing gradient gel electrophoresis to characterize a single nucleotide substitution in the apolipoprotein B gene. This mutation affects amino acid 4311 of the protein and converts asparagine to serine. It was found in 24% of the 81 unrelated individuals analyzed. Moreover, another mutation was detected by sequencing in a single individual.

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