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The isolation of poly(3-hydroxybutyrate) from recombinant Escherichia coli XL1-blue using the digestion method

โœ Scribed by Yu-Chiang Peng; Chi-Wei Lo; Ho-Shing Wu

Book ID
112051890
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
244 KB
Volume
91
Category
Article
ISSN
0008-4034

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โœ Jong-il Choi; Sang Yup Lee ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 223 KB ๐Ÿ‘ 2 views

A simple method for the recovery of microbial poly(3-hydroxybutyrate) [P(3HB)] from recombinant Escherichia coli harboring the Ralstonia eutropha PHA biosynthesis genes was developed. Various acids (HCl, H 2 SO 4 ), alkalies (NaOH, KOH, and NH 4 OH), and surfactants (dioctylsulfosuccinate sodium sal