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Construction of plasmids, estimation of plasmid stability, and use of stable plasmids for the production of poly(3-hydroxybutyric acid) by recombinant Escherichia coli

โœ Scribed by S.Y. Lee; K.S. Yim; H.N. Chang; Y.K. Chang


Book ID
119132133
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
676 KB
Volume
32
Category
Article
ISSN
0168-1656

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A technique is presented by which mutations can be introduced into the Escherichia coli chromosome by gene replacement between the chromosome and a plasmid carrying the mutant gene. The segregational instability of plasmids in E. coli is used with high efficiency to isolate E. coli mutants. The meth