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Construction and evaluation of a plasmid vector for the expression of recombinant lipoproteins in Escherichia coli

โœ Scribed by Paul A. Cullen; Miranda Lo; Dieter M. Bulach; Stuart J. Cordwell; Ben Adler


Book ID
114122034
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
301 KB
Volume
49
Category
Article
ISSN
0147-619X

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โœ Kiel, J. A. K. W. ;Vossen, J. P. M. J. ;Venema, G. ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 904 KB

A technique is presented by which mutations can be introduced into the Escherichia coli chromosome by gene replacement between the chromosome and a plasmid carrying the mutant gene. The segregational instability of plasmids in E. coli is used with high efficiency to isolate E. coli mutants. The meth