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Expression of Mouse Uterine Peptidylarginine Deiminase in Escherichia coli: Construction of Expression Plasmid and Properties of the Recombinant Enzyme

✍ Scribed by I. Ohsugi; H. Takahara; M. Shiraiwa; K. Sugawara

Book ID
115565473
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
587 KB
Volume
317
Category
Article
ISSN
0003-9861

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The lysA gene of Escherichia coli has been cloned from a lambda transducing phage on various plasmids, present in different copy numbers in bacterial cells. Synthesis of the product of this gene, diaminopimelate (DAP)-decarboxylase, and its regulation have been studied. Expression does not follow a