Five nitrate reductase-deficient mutants of tomato were isolated from an M2 population after ethylmethanesulphonate (EMS) seed treatment by means of selection for chlorate resistance. All mutations were monogenic and recessive and complementation analysis revealed that they were non-allelic. Biochem
The isolation and characterization of gibberellin-deficient mutants in tomato
β Scribed by M. Koornneef; T. D. G. Bosma; C. J. Hanhart; J. H. Veen; J. A. D. Zeevaart
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 740 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0040-5752
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β¦ Synopsis
In tomato, nine independent EMS-induced mutants representing recessive mutations at three different loci (gib-1, gib-2, and gib-3) were isolated. Six of these have an almost absolute gibberellin requirement for seed germination and elongation growth. In addition, the leaves are darker green, smaller, and changed in structure as compared to wild type. The three other mutants, which germinate without GA, are allelic to specific, nongerminating mutants and have less severe mutant characteristics. The respective loci are situated on three different chromosomes. The genes identified by these mutants control steps in gibberellin biosynthesis, as endogenous gibberellins are strongly reduced.
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