𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The isolation and characterization of gibberellin-deficient mutants in tomato

✍ Scribed by M. Koornneef; T. D. G. Bosma; C. J. Hanhart; J. H. Veen; J. A. D. Zeevaart


Publisher
Springer
Year
1990
Tongue
English
Weight
740 KB
Volume
80
Category
Article
ISSN
0040-5752

No coin nor oath required. For personal study only.

✦ Synopsis


In tomato, nine independent EMS-induced mutants representing recessive mutations at three different loci (gib-1, gib-2, and gib-3) were isolated. Six of these have an almost absolute gibberellin requirement for seed germination and elongation growth. In addition, the leaves are darker green, smaller, and changed in structure as compared to wild type. The three other mutants, which germinate without GA, are allelic to specific, nongerminating mutants and have less severe mutant characteristics. The respective loci are situated on three different chromosomes. The genes identified by these mutants control steps in gibberellin biosynthesis, as endogenous gibberellins are strongly reduced.


πŸ“œ SIMILAR VOLUMES


Isolation and characterization of nitrat
✍ Schoenmakers, Herman C. H. ;Koornneef, Maarten ;Alefs, Sjefke J. H. M. ;Gerrits, πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 1013 KB

Five nitrate reductase-deficient mutants of tomato were isolated from an M2 population after ethylmethanesulphonate (EMS) seed treatment by means of selection for chlorate resistance. All mutations were monogenic and recessive and complementation analysis revealed that they were non-allelic. Biochem

Isolation and characterization ofKluyver
✍ QueirΓ³s, O.; Casal, M.; Althoff, S.; Moradas-Ferreira, P.; LeΓ£o, C. πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 136 KB

In malic acid-grown cells of the strains ATCC 10022 and KMS3 of Kluyveromyces marxianus the transport of malic acid occurred by a malate-proton symport, which accepted -malic, -malic, succinic and fumaric acids, but not tartaric, malonic or maleic acids. The system was inducible and subjected to g

Isolation and characterization of Strept
✍ Tsai, Jane F. -Y. ;Chen, Carton W. πŸ“‚ Article πŸ“… 1987 πŸ› Springer 🌐 English βš– 890 KB

Plasmid pIF132 containing two direct repeats of the mel (melanin) sequence was used to monitor intraplasmid recombination. Five mutants of Streptomyces lividans TK64 deficient in intraplasmid recombination were isolated. Four contained additional defects in aerial mycelium formation, pigmentation, a

Gibberellin-induced changes in the trans
✍ Steven E. Jacobsen; Lifang Shi; Zhanguo Xin; Neil E. Olszewski πŸ“‚ Article πŸ“… 1994 πŸ› Springer-Verlag 🌐 English βš– 783 KB

The gibl mutant of tomato (Lycopersicon esculentum Mill.) is deficient in endogenous gibberellins and exhibits phenotypes including extreme dwarfism, reduced germination, and abnormal flower development, which are reversed by the application of gibberellic acid (GA3). Previous work has demonstrated