𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Isolation and characterization of Streptomyces lividans mutants deficient in intraplasmid recombination

✍ Scribed by Tsai, Jane F. -Y. ;Chen, Carton W.

Publisher
Springer
Year
1987
Tongue
English
Weight
890 KB
Volume
208
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

Plasmid pIF132 containing two direct repeats of the mel (melanin) sequence was used to monitor intraplasmid recombination. Five mutants of Streptomyces lividans TK64 deficient in intraplasmid recombination were isolated. Four contained additional defects in aerial mycelium formation, pigmentation, and nutrient requirements; among these two showed extensive amplification of chromosomal sequences. Mutant JT46 had no pleiotropic defects but had the most severe blockage in recombination. Only one of the mutants was slightly more sensitive to UV and two were slightly more sensitive to mitomycin C. Plasmid pWCL1 (containing pIJ702, pUC12, and HBVsAg sequences; Lee et al. 1986) could not stably replicate in TK64 without spontaneous deletions. In contrast the mutant JT46 maintained the integrity of pWCL1 much more stably.

πŸ“œ SIMILAR VOLUMES

The isolation and characterization of gi
The isolation and characterization of gibberellin-deficient mutants in tomato
✍ M. Koornneef; T. D. G. Bosma; C. J. Hanhart; J. H. Veen; J. A. D. Zeevaart πŸ“‚ Article πŸ“… 1990 πŸ› Springer 🌐 English βš– 740 KB

In tomato, nine independent EMS-induced mutants representing recessive mutations at three different loci (gib-1, gib-2, and gib-3) were isolated. Six of these have an almost absolute gibberellin requirement for seed germination and elongation growth. In addition, the leaves are darker green, smaller

Isolation and characterization ofKluyver
Isolation and characterization ofKluyveromyces marxianus mutants deficient in malate transport
✍ QueirΓ³s, O.; Casal, M.; Althoff, S.; Moradas-Ferreira, P.; LeΓ£o, C. πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 136 KB

In malic acid-grown cells of the strains ATCC 10022 and KMS3 of Kluyveromyces marxianus the transport of malic acid occurred by a malate-proton symport, which accepted -malic, -malic, succinic and fumaric acids, but not tartaric, malonic or maleic acids. The system was inducible and subjected to g

Isolation and characterization of mutant
Isolation and characterization of mutants of Aspergillus niger deficient in extracellular proteases
✍ Mattern, Ineke E. ;van Noort, Johannes M. ;van den Berg, Paul ;Archer, David B. πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 690 KB

In the present study, the extracellular protease activity in a strain of the filamentous fungus Aspergillus niger was investigated and mutant strains deficient in the production of extracellular proteases were isolated. The major protease, which is responsible for 80-85% of the total activity, is as