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Isolation and characterization of nitrate reductase-deficient mutants in tomato (Lycopersicon esculentum Mill.)

✍ Scribed by Schoenmakers, Herman C. H. ;Koornneef, Maarten ;Alefs, Sjefke J. H. M. ;Gerrits, Wendy F. M. ;van der Kop, Dianne ;Chérel, Isabelle ;Caboche, Michel

Publisher
Springer
Year
1991
Tongue
English
Weight
1013 KB
Volume
227
Category
Article
ISSN
0026-8925

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✦ Synopsis

Five nitrate reductase-deficient mutants of tomato were isolated from an M2 population after ethylmethanesulphonate (EMS) seed treatment by means of selection for chlorate resistance. All mutations were monogenic and recessive and complementation analysis revealed that they were non-allelic. Biochemical and molecular characterization of these mutants showed that four of them are cofactor mutants while one is an apoenzyme mutant.

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