𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The interaction of non-transforming Epstein-Barr virus (EBV) with cell-associated EBV DNA in superinfected lymphoblastoid cell lines

✍ Scribed by Jay Stoerker; Yoshihiro Yajima; Ronald Glaser

Publisher
John Wiley and Sons
Year
1982
Tongue
French
Weight
798 KB
Volume
30
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Two human lymphoblastoid cell lines were established by the transformation of human cord‐blood lymphocytes with transforming Epstein‐Barr virus (EBV). One cell line (HLB‐R1) was established with EBV obtained after the superinfection of Raji cells with HR‐1 EBV and the other (HLB‐B1) was established from B95‐8 EBV‐infected human cord‐blood lymphocytes. Both the HLB‐R1 and HLB‐B1 lines were susceptible to superinfection with HR‐1 EBV. We found that EBV DNA was replicated in the superinfected cell lines and that transforming EBV was produced in both the HLB‐B1 and HLB‐R1 cells. The average titer of transforming EBV obtained in the HR‐1 EBV superinfected HLB‐B1 and HLB‐R1 cell lines was 10^4^ transforming units (TU)/ml, whereas the average titers of transforming EBV obtained by the superinfection of Raji cells was 10 TU/ml. Epstein‐Barr virus capable of inducing early antigen (EA) in superinfected Raji cells (lytic virus) was not detected in any transforming virus preparation. Restriction enzyme digestion patterns of virus DNA isolated from HR‐1 and B95‐8 cells, as well as from superinfected cells, were compared. The EBV DNA that was replicated in the superinfected HLB‐R1 and HLB‐B1 cell lines showed a more complex pattern. Our data suggest that recombination between input HR‐1 EBV DNA and latent cell‐associated EBV DNA occurs. Presumably this recombination results in a change in the biological properties of the newly synthesized virus.

📜 SIMILAR VOLUMES

Sensitivity of Epstein-Barr virus (EBV)
Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus
✍ George Klein; Laszlo Dombos; Balwant Gothoskar 📂 Article 📅 1972 🏛 John Wiley and Sons 🌐 French ⚖ 928 KB

## Abstract Twenty‐nine lymphoblastoid lines and one IgE‐producing myeloma line of human origin were exposed to Epstein‐Barr virus (EBV) concentrates __in vitro__. The adsorption of the virus to the outer cell membrane was assessed by counting the number of direct membrane fluorescence‐positive cel

Tumorigenicity of Epstein-barr virus (EB
Tumorigenicity of Epstein-barr virus (EBV)-transformed lymphoid line cells in autologous squirrel monkeys
✍ Wolfgang Leibold; Tom D. Flanagan; Maria Andersson; Maurice Dalens; George Klein 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 French ⚖ 773 KB

## Abstract Eight squirrel monkeys (Saimiri sciureus) challenged with EBV or EBV‐transformed SLCL were naturally or experimentally infected with Plasmodium knowlesi or Pl. brasilianum. Most of the animals had been splenectomized and unilaterally nephrectomized. Three of these monkeys received one d

Detection of Epstein-Barr virus (EBV)-as
Detection of Epstein-Barr virus (EBV)-associated nuclear antigen in human lymphoblastoid cell lines by means of an 125I-IgG-binding assay
✍ T. D. K. Brown; I. Ernberg; E. W. Lamon; G. Klein 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 572 KB

## Abstract The elution at low pH of human EBV‐positive ^125^I‐IgG bound to preparations of EBV‐carrying non‐producer lymphoblastoid cell lines has been investigated. Specific binding was demonstrated. A number of approaches were used to confirm the specificity. The stoichiometry of binding and elu

The action of dna antagonists on epstein
The action of dna antagonists on epstein-barr virus (EBV)-associated early antigen (EA) in burkitt lymphoma lines
✍ Lajos Gergely; George Klein; Ingemar Ernberg 📂 Article 📅 1971 🏛 John Wiley and Sons 🌐 French ⚖ 651 KB

## Abstract The effect of two DNA antagonists (IUDR and Ara C) on EBV‐associated antigens was studied in two BL‐derived carrier culture lines (P3HR‐1 and Onesmas). Both drugs led to an accumulation of the early antigen (EA) from 2% in the untreated to 5–40% in the treated cultures. Ara C blocked th

Abortive expression of the epstein-barr
Abortive expression of the epstein-barr virus (EBV) cycle in a variety of EBV DNA-containing cell lines, as reflected by nucleic acid hybridization In situ
✍ Martin H. Moar; George Klein 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 748 KB

## Abstract A variety of Epstein‐Barr virus (EBV) DNA‐containing cell lines have been tested for the expression of the EBV‐associated antigens EBNA (nuclear antigen), EA (early antigen), and VCA (viral capsid antigen), and for the presence of cells containing disproportionate amounts of EBV DNA. Th

Heterogeneity of epstein-barr virus. IV.
Heterogeneity of epstein-barr virus. IV. Induction of a specific antigen by EBV from two transformed marmoset cell lines in ramos cells
✍ K.-O. Fresen; M.-S. Cho; H. Zur Hausen 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 French ⚖ 581 KB

## Abstract Infection of cells of the EBV‐genome‐negative human B‐lymphoma Ramos line with viral isolates obtained from two EBV‐transformed marmoset cell lines (B95/8; Nyevu) resulted in the induction of a nuclear antigen (RAM‐ag) apparently different from other EBV‐associated antigen complexes. Th