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Detection of Epstein-Barr virus (EBV)-associated nuclear antigen in human lymphoblastoid cell lines by means of an 125I-IgG-binding assay

✍ Scribed by T. D. K. Brown; I. Ernberg; E. W. Lamon; G. Klein

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
572 KB
Volume
13
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The elution at low pH of human EBV‐positive ^125^I‐IgG bound to preparations of EBV‐carrying non‐producer lymphoblastoid cell lines has been investigated. Specific binding was demonstrated. A number of approaches were used to confirm the specificity. The stoichiometry of binding and elution was studied. The sub‐cellular location of the EBV‐associated antigen (s) detected by this method was shown to be predominantly nuclear. This confirms other studies demonstrating the presence of a nuclear antigen in non‐producer lymphoblastoid cell lines carrying the EBV genome.

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Studies of epstein-barr virus (ebv)-asso
Studies of epstein-barr virus (ebv)-associated nuclear antigen. I. Assay in human lymphoblastoid cell lines by direct and indirect determination of 125i-igg binding
✍ T. D. K. Brown; I. Ernberg; G. Klein 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 French ⚖ 684 KB

## Abstract A quantitative assay for EBNA in human lymphoblastoid cell lines has been developed. The assay employs EBNA‐positive and ‐negative ^125^I‐IgG preparations as reagents and can be used in a direct or indirect manner. EBNA specificity has been demonstrated in a number of ways. The antigeni