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Studies of epstein-barr virus (ebv)-associated nuclear antigen. I. Assay in human lymphoblastoid cell lines by direct and indirect determination of 125i-igg binding

✍ Scribed by T. D. K. Brown; I. Ernberg; G. Klein

Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
684 KB
Volume
15
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

A quantitative assay for EBNA in human lymphoblastoid cell lines has been developed. The assay employs EBNA‐positive and ‐negative ^125^I‐IgG preparations as reagents and can be used in a direct or indirect manner. EBNA specificity has been demonstrated in a number of ways. The antigenic relationship between EBNA present in Raji cells and in a number of other human lymphoblastoid cell lines of diverse origins and between the cell‐associated antigen present in FT Raji cells and that present in isolated Raji nuclei has been studied. The possibility of carrying out blocking titrations for anti‐EBNA determination has been demonstrated and the effect of glutaraldehyde and formaldehyde upon antigenic activity in FT Raji cells has been studied.

📜 SIMILAR VOLUMES

Detection of Epstein-Barr virus (EBV)-as
Detection of Epstein-Barr virus (EBV)-associated nuclear antigen in human lymphoblastoid cell lines by means of an 125I-IgG-binding assay
✍ T. D. K. Brown; I. Ernberg; E. W. Lamon; G. Klein 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 572 KB

## Abstract The elution at low pH of human EBV‐positive ^125^I‐IgG bound to preparations of EBV‐carrying non‐producer lymphoblastoid cell lines has been investigated. Specific binding was demonstrated. A number of approaches were used to confirm the specificity. The stoichiometry of binding and elu