𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Tumorigenicity of Epstein-barr virus (EBV)-transformed lymphoid line cells in autologous squirrel monkeys

✍ Scribed by Wolfgang Leibold; Tom D. Flanagan; Maria Andersson; Maurice Dalens; George Klein; Gunnel Huldt; Dennis H. Wright; Alister Voller

Publisher
John Wiley and Sons
Year
1976
Tongue
French
Weight
773 KB
Volume
17
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Eight squirrel monkeys (Saimiri sciureus) challenged with EBV or EBV‐transformed SLCL were naturally or experimentally infected with Plasmodium knowlesi or Pl. brasilianum. Most of the animals had been splenectomized and unilaterally nephrectomized. Three of these monkeys received one dose of 6 to 12 × 10^8^ autologous SLCL. These lines were derived from saimiri lymphoid cells permanently transformed by B‐EBV in vitro. All three animals developed multiple undifferentiated malignant lymphomas and died 8 to 10 days post inoculation. Necropsy tumor specimens were EBNA‐positive and contained 7 to 21 EBV genome equivalents per cell. EBNA‐ and EA‐positive SLCL were established in vitro from four tumor explants of two monkeys. These results demonstrate that in vitro EBV‐transformed SLCL are able to cause tumor formation in autologous squirrel monkeys. Five monkeys received high transforming doses of B‐EBV or S‐EBV, derived from one of the tumorigenic SLCL. None of the animals developed any sign of tumor formation during an observation period of up to 130 days. Three of these monkeys showed no detectable EBV‐related seroconversion while the sera of two monkeys had become anti‐EBNA‐positive when tested at days 28 and 130 respectively post inoculation. Two additional monkeys received neither EBV nor SLCL. They showed no clinical evidence of tumor development or spontaneous seroconversion over a period of more than 1 year.

📜 SIMILAR VOLUMES

The interaction of non-transforming Epst
The interaction of non-transforming Epstein-Barr virus (EBV) with cell-associated EBV DNA in superinfected lymphoblastoid cell lines
✍ Jay Stoerker; Yoshihiro Yajima; Ronald Glaser 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 French ⚖ 798 KB

## Abstract Two human lymphoblastoid cell lines were established by the transformation of human cord‐blood lymphocytes with transforming Epstein‐Barr virus (EBV). One cell line (HLB‐R1) was established with EBV obtained after the superinfection of Raji cells with HR‐1 EBV and the other (HLB‐B1) was

Establishment of human cytotoxic T-cell
Establishment of human cytotoxic T-cell lines specific for epstein-barr virus-transformed autologous cells
✍ Dr. Kazuo Sugamura; Yuetsu Tanaka; Yorio Hinuma 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 French ⚖ 534 KB

## Abstract EBV‐specific cytotoxic T cells (Tc)^1^ induced __in vitro__ have continuously proliferated __in vitro__ for over 9 months. The long‐term maintenance of the Tc growth was dependent on periodic supplementation of both irrediated EBV‐transformed autologous lymphoblastoid cell line (LCL) ce

Characterization of lymphoid cell lines
Characterization of lymphoid cell lines established from multiple epstein-barr virus (EBV)-induced lymphomas in a cotton-topped marmoset
✍ Harvey Rabin; Russell H. Neubauer; Ralph F. Hopkins III; Barnet M. Levy 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 675 KB

## Abstract Inoculation of a cotton‐topped marmoset with cell‐free EBV, strain B95‐8, resulted in the induction of lymphomas in liver, spleen, and mesenteric lymph nodes. Continous suspension cell lines were established from tumor tissue from each of these three sites. Cells of each line were posit

Column separation of viral capsid antige
Column separation of viral capsid antigen (vca)-positive cells from vca-negative cells in an epstein-barr virus (ebv)-producing lymphoid line
✍ Tina Dalianis; George Klein; Birger Andersson 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 642 KB

## Abstract Virus producing, VCA (viral capsid antigen)‐positive cells could be selectively removed from Epstein‐Barr virus (EBV)‐carrying, virus‐producer P3HR‐1 cultures by two different methods of column passage. In the first, the virus‐producing cells were covered with human EBV antibody and sub

Epstein-Barr virus (EBV) gene expression
Epstein-Barr virus (EBV) gene expression in lymphoid B cells during acute infectious mononucleosis (IM) and clonality of the directly growing cell lines
✍ Nongnit Laytragoon-Lewin; Fu Chen; Javier Avila-Carino; George Klein; Håkan Mell 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 269 KB 👁 2 views

We examined the patterns of viral gene expression in acute infectious mononucleosis (IM) patients and the clonality of the directly growing EBV-carrying cell lines. Both low- and high-density EBV-carrying B cells obtained from the patients' tonsils expressed EBNA1, EBNA2 and LMP1. Like LCLs and immu