✦ LIBER ✦

The interaction of Hoechst 33258 and BrdU substituted DNA in the formation of sister chromatid exchanges

✍ Scribed by D. G. Stetka; A. V. Carrano

Publisher: Springer
Year: 1977
Tongue: English
Weight: 658 KB
Volume: 63
Category: Article
ISSN: 0009-5915
DOI: 10.1007/bf00292939

No coin nor oath required. For personal study only.

✦ Synopsis

Sister chromatid exchanges (SCEs) are induced in cultured Chinese hamster cells by treatment with 5-bromodeoxyuridine (BrdU) or with Hoechst 33258 (H33258) plus BrdU. The SCE frequencies depend upon the number of H33258 molecules available per cell (or per base pair) and the number of brdU molecules available per cell, and not solely upon molarity. In addition, H33258 and BrdU act synergistically to induce SCEs. At low BrdU concentrations H 33258 induces very few SCEs. At high BrdU concentrations and similar concentrations of H33258, however, SCE frequencies are significantly increased. SCE frequencies decrease with time in successively harvested cells because of the depletion of H33258 from the medium due to DNA binding.

📜 SIMILAR VOLUMES

Synergistic action of cysteamine and Brd

Synergistic action of cysteamine and BrdU-substituted DNA in the induction of sister chromatid exchanges

✍ G. Speit; M. Wolf; W. Vogel 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 772 KB

The effect of different BrdU-concentrations on the cysteamine-induced SCE-rate was investigated in V-79 Chinese hamster cell monolayer cultures. Both cysteamine and its auto-oxidation product cystamine act synergistically with BrdU in the induction of SCEs. A given concentration of these substances

Different effects of 33258 Hoechst and D

Different effects of 33258 Hoechst and DAPI in fluorescent staining of sister chromatids differentially substituted with bromodeoxyuridine

✍ C. H. C. M. Buys; A. Y. Veen 📂 Article 📅 1982 🏛 Springer 🌐 English ⚖ 935 KB

Persistence of DNA lesions and the cytol

Persistence of DNA lesions and the cytological cancellation of sister chromatid exchanges

✍ J. B. Schvartzman; V. J. Goyanes; A. Campos; A. M. Lage; C. Veiras; M. C. Silva; 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 552 KB

The ability of UV light, mitomycin C and ionizing radiation to induce the formation of sister chromatid exchanges (SCEs) at the same locus in successive cell generations was investigated in human lymphocytes. Cells were exposed to the DNA damaging agents after they had completed their first round of

Electrochemical Studies for the Interact

Electrochemical Studies for the Interaction of DNA with an Irreversible Redox Compound – Hoechst 33258

✍ Wang Sufen; Peng Tuzhi; Catherine F. Yang 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 97 KB

Distribution of sister chromatid exchang

Distribution of sister chromatid exchanges in the euchromatin and heterochromatin of the Indian muntjac

✍ A. V. Carrano; Sheldon Wolff 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 1017 KB

The frequency of sister chromatid exchanges (SCEs) was determined for the chromosomes (except Y2) of the Indian muntjac stained by the fluorescence plus Giemsa (FPG) or harlequin chromosome technique. The relative DNA content of each of the chromosomes was also measured by scanning cytophotometry. A

Deficiency of arginine and lysine causes

Deficiency of arginine and lysine causes increase in the frequency of sister chromatid exchanges

✍ W. Schempp; W. Krone 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 199 KB

An increase in the rate of sister chromatid exchanges (SCE) was found when V79 Chinese hamster cells were exposed to increasingly severe degrees of arginine and lysine deficiency. The data suggest a possible function of chromosomal proteins, and of histones in particular, in the maintenance of the l