✦ LIBER ✦

Persistence of DNA lesions and the cytological cancellation of sister chromatid exchanges

✍ Scribed by J. B. Schvartzman; V. J. Goyanes; A. Campos; A. M. Lage; C. Veiras; M. C. Silva; S. Ramos

Publisher: Springer
Year: 1985
Tongue: English
Weight: 552 KB
Volume: 92
Category: Article
ISSN: 0009-5915
DOI: 10.1007/bf00327239

No coin nor oath required. For personal study only.

✦ Synopsis

The ability of UV light, mitomycin C and ionizing radiation to induce the formation of sister chromatid exchanges (SCEs) at the same locus in successive cell generations was investigated in human lymphocytes. Cells were exposed to the DNA damaging agents after they had completed their first round of DNA replication, and SCEs were examined at the third division in chromosomes that had been differentially stained three ways. Although some of these treatments induced long-lived lesions that increased the frequency of SCEs in successive cell generations, none of the lesions led to the formation of consecutive SCEs at the same locus in successive cell generations. This observation seriously challenges the hypothesis that SCE cancellation results as a consequence of persistence of the lesions induced by these agents.

📜 SIMILAR VOLUMES

Sister chromatid exchanges in patients w

Sister chromatid exchanges in patients with precancerous and cancerous lesions of cervix uteri

✍ V. V. V. S. Murty; A. B. Mitra; U. K. Luthra; I. P. Singh 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 541 KB

Do the frequencies of sister chromatid e

Do the frequencies of sister chromatid exchanges in endoreduplieated mitoses provide a measure for lesion persistence and repair?

✍ Günter Speit; Walther Vogel; Karl Mehnert 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 363 KB

Synergistic action of cysteamine and Brd

Synergistic action of cysteamine and BrdU-substituted DNA in the induction of sister chromatid exchanges

✍ G. Speit; M. Wolf; W. Vogel 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 772 KB

The effect of different BrdU-concentrations on the cysteamine-induced SCE-rate was investigated in V-79 Chinese hamster cell monolayer cultures. Both cysteamine and its auto-oxidation product cystamine act synergistically with BrdU in the induction of SCEs. A given concentration of these substances

The interaction of Hoechst 33258 and Brd

The interaction of Hoechst 33258 and BrdU substituted DNA in the formation of sister chromatid exchanges

✍ D. G. Stetka; A. V. Carrano 📂 Article 📅 1977 🏛 Springer 🌐 English ⚖ 658 KB

Sister chromatid exchanges (SCEs) are induced in cultured Chinese hamster cells by treatment with 5-bromodeoxyuridine (BrdU) or with Hoechst 33258 (H33258) plus BrdU. The SCE frequencies depend upon the number of H33258 molecules available per cell (or per base pair) and the number of brdU molecules

Inhibitors of DNA synthesis induce siste

Inhibitors of DNA synthesis induce sister chromatid exchanges at the early S phase of the cell cycle

✍ G. Rainaldi; M. R. Sessa; T. Mariani 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 804 KB

To investigate the origin of sister chromatid exchanges (SCEs) induced by inhibitors of DNA synthesis, V79/AP4 Chinese hamster cells were treated with aphidicolin, 1-beta-D-arabinofuranosylcytosine, and thymidine. At the end of the treatments we determined both the distribution of the cells in the v

Distribution of sister chromatid exchang

Distribution of sister chromatid exchanges in the euchromatin and heterochromatin of the Indian muntjac

✍ A. V. Carrano; Sheldon Wolff 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 1017 KB

The frequency of sister chromatid exchanges (SCEs) was determined for the chromosomes (except Y2) of the Indian muntjac stained by the fluorescence plus Giemsa (FPG) or harlequin chromosome technique. The relative DNA content of each of the chromosomes was also measured by scanning cytophotometry. A