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Synergistic action of cysteamine and BrdU-substituted DNA in the induction of sister chromatid exchanges

✍ Scribed by G. Speit; M. Wolf; W. Vogel

Publisher: Springer
Year: 1980
Tongue: English
Weight: 772 KB
Volume: 81
Category: Article
ISSN: 0009-5915
DOI: 10.1007/bf00368156

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✦ Synopsis

The effect of different BrdU-concentrations on the cysteamine-induced SCE-rate was investigated in V-79 Chinese hamster cell monolayer cultures. Both cysteamine and its auto-oxidation product cystamine act synergistically with BrdU in the induction of SCEs. A given concentration of these substances produces a low SCE-frequency at low BrdU-concentrations -- but the incidence of SCEs is significantly increased at increased BrdU-concentrations. -- Using one-cycle substitution experiments and the determination of the relative level of substitution by means of 3HBrdU-incorporation, this synergism was shown to depend on the BrdU incorporated in the DNA and on the extent to which this incorporation takes place.

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