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The influence of chamber kind and plate type on TLC separation of saponins

✍ Scribed by Amarowicz, R. ;Shimoyamada, M. ;Okubo, K.

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
153 KB
Volume
36
Category
Article
ISSN
0027-769X

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✦ Synopsis

Thin layer chromatography has been applied by several workers for the qualitative and quantitative estimation of saponins in plant extracts [2][3][4][5][8][9][10]. Numerous developing systems are applied for saponin separation. Of these the two that appear to be most often used are n-butanolethanol -33% NH,OH (7 : 2 : 5) and chloroformmethanolwater ( 65: 35 : 10) [lo]. Visualization spray includes CARR-PRICE reagent [4], LIBERMANN-BURCHARD reagent [9], phosphortungstic acid [5] and H2S0, solutions [3, 101. Quantitative analysis applies TLC-spectrophotometry [6] and TLC-densitometry [8].

The aim of the work was to compare the separations of selected saponins by thin-layer chromatography depending on the kind of applied chamber (classical or sandwich) and depending on the type of silica-gel plate (TLC or HPTLC).

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