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The influence of cell population density and serum on the (Na+K)-ATPase of 3T3 cells

✍ Scribed by Brian McCaldin; Effendi M. Tenang

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 211 KB
Volume: 123
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041230323

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✦ Synopsis

Abstract

The (Na^+^K)‐ATPase of 3T3 cells has been measured as a function of culture density and the type of serum in which the cells were grown. When 3T3 cells were grown in medium containing 10% newborn calf serum their (Na^+^K)‐ATPase activity increased as the culture density increased and the cells became quiescent at densities of about 17.5 × 10^4^ cells/cm^2^. When these cells are subcultured into medium containing 10% foetal bovine serum the activity of the enzyme decreases as the culture density increases and the cells attain final densities of about 5 × 10^4^ cells/cm^2^.

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