𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The induction of pyruvate kinase synthesis by heat shock inXenopus laevis embryos

✍ Scribed by Marsden, M. ;Nickells, R. W. ;Kapoor, M. ;Browder, L. W.

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
689 KB
Volume
14
Category
Article
ISSN
0192-253X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Heat‐shocked Xenopus embryos have an unusually complex heat shock response. The dominant heat shock protein (Hsp) has a relative molecular mass (M~r~) of 62,000 D (Hsp62). Affinity‐purified IgGs against the glycolytic enzyme pyruvate kinase (PK; EC 2.7.1.40) specifically immunoprecipitated Hsp62 from extracts of embryos that had been heat‐shocked at 37°C for 30 min. Thus, Hsp62 and pyruvate kinase are immunologically cross‐reacting. Electrophoretic separation of PK isoforms suggests that heat‐shocked Xenopus embryos increase synthesis of an isoform of PK. Thermal denaturation studies suggest that this isoform has enhanced thermal stability. The identification of PK as an Hsp is discussed within the context of a physiological requirement for elevated levels of anaerobic glycolysis in heatstressed cells as a vital component of the acquisition of thermotolerance. © 1993Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Induction of triploidy inXenopus laevis
Induction of triploidy inXenopus laevis by heat shocking of the egg before and after fertilization
✍ Cassidy, Donna M. ;Blackler, Antonie W. 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 491 KB

## Abstract Eggs of the frog __Xenopus laevis__ have been subjected to a temperature shock before or after fertilization. The shock provokes triploidy through suppression of elimination of the second polar body; application of the shock within ten minutes of fertilization was associated with triplo

Spatial pattern of constitutive and heat
Spatial pattern of constitutive and heat shock-induced expression of the small heat shock protein gene family, hsp30, inXenopus laevis tailbud embryos
✍ Lang, Lisa ;Miskovic, Dragana ;Fernando, Pasan ;Heikkila, John J. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 208 KB 👁 2 views

We employed whole-mount in situ hybridization and immunohistochemistry to study the spatial pattern of hsp30 gene expression in normal and heatshocked embryos during Xenopus laevis development. Our findings revealed that hsp30 mRNA accumulation was present constitutively only in the cement gland of

Determination of the rate of rRNA synthe
Determination of the rate of rRNA synthesis inXenopus laevis triploid embryos produced by low-temperature treatment
✍ Tashiro, K. ;Misumi, Y. ;Shiokawa, K. ;Yamana, K. 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 560 KB

## Abstract Triploid embryos of __Xenopus laevis__ were obtained by cold‐temperature shocking of the fertilized eggs, and the rate of the ribosonial RNA (rRNA) synthesis was determined for comparison with that in diploid embryos. For this purpose, both triploid and diploid embryos were dissociated