Induction of triploidy inXenopus laevis by heat shocking of the egg before and after fertilization
✍ Scribed by Cassidy, Donna M. ;Blackler, Antonie W.
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 491 KB
- Volume
- 211
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Eggs of the frog Xenopus laevis have been subjected to a temperature shock before or after fertilization. The shock provokes triploidy through suppression of elimination of the second polar body; application of the shock within ten minutes of fertilization was associated with triploid frequencies to 90%. All series in which eggs were shocked after fertilization manifested some abnormal development in the surviving eggs, the greatest abnormality frequency correlated with the period of greatest sensitivity for the induction of triploidy. Up to 50% triploidy was induced in the eggs of certain females when the temperature shock was applied before fertilization. In these series, the capacity for normal development was not appreciably lowered by the treatment. A comparison is made between thermal and pressure techniques for the induction of triploidy.