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The incorporation of S35 methionine by the uterus and ova of the mouse

โœ Scribed by Greenwald, Gilbert S. ;Everett, Newton B.


Publisher
John Wiley and Sons
Year
1959
Tongue
English
Weight
812 KB
Volume
134
Category
Article
ISSN
0003-276X

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Five-day-old mouse blastocysts were transferred into the oviducts of recipients on the second day oโ‚ฌ pregnancy. S ~S methionine was then injected into the recipients and the blastocysts and native 2-celled eggs were recovered six hours later. Radioautographs reveal that the blastocysts incorporate 5

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Incorporation of 35S methionine by mouse blastocysts in vivo, as detected by radioautography, provides a presumptive measure of the blastocysts' protein synthesizing activity. Normally, there is a marked increase in 85S methionine incorporation between 95 and 99 hours post coitum. To determine whet