A comparison of thein vivo incorporation of S35 methionine by two-celled mouse eggs and blastocysts
✍ Scribed by Weitlauf, Harry M. ;Greenwald, Gilbert S.
- Publisher
- John Wiley and Sons
- Year
- 1967
- Tongue
- English
- Weight
- 430 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0003-276X
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✦ Synopsis
Five-day-old mouse blastocysts were transferred into the oviducts of recipients on the second day o€ pregnancy. S ~S methionine was then injected into the recipients and the blastocysts and native 2-celled eggs were recovered six hours later. Radioautographs reveal that the blastocysts incorporate 5 3 5 methioniiie while exposed to the tubal environment to the same degree that they would in the uterus. However, the 2-celled eggs in the same oviducal environment incorporate little or no methionine. It is therefore concluded that the difference in the incorporation o f S3: methionine is due to maturational changes in the blastocyst rather than to a deficiency of the labelled amino acid in the tubal lumen.