✦ LIBER ✦
Influence of estrogen and progesterone on the incorporation of35S methionine by blastocysts in ovariectomized mice
✍ Scribed by Weitlauf, Harry M. ;Greenwald, Gilbert S.
- Publisher
- John Wiley and Sons
- Year
- 1968
- Tongue
- English
- Weight
- 564 KB
- Volume
- 169
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Incorporation of 35S methionine by mouse blastocysts in vivo, as detected by radioautography, provides a presumptive measure of the blastocysts' protein synthesizing activity. Normally, there is a marked increase in 85S methionine incorporation between 95 and 99 hours post coitum.
To determine whether or not ovarian hormones influence 35S methionine incorporation by the blastocysts, mice were ovariectomized on day 4 of pregnancy and injected with progesterone and/or estradiol 17-p and given a single injection of 3% methionine.