✦ LIBER ✦

The in vitro cytotoxicity of asbestos fibers: I. P388D1 cells

✍ Scribed by A. Wright; H. Cowie; I. P. Gormley; Dr. J. M. G. Davis

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 870 KB
Volume: 9
Category: Article
ISSN: 0271-3586
DOI: 10.1002/ajim.4700090409

No coin nor oath required. For personal study only.

✦ Synopsis

In this study, the cytotoxicity of 13 fibrous samples of known fiber number and dimensions has been established in P388D1 cells. The cells were exposed in vitro to dust concentrations of 10 or 50 pglml and, after incubation for 24 or 48 hours, any changes in cellular viability, lactate dehydrogenase, and glucosaminidase levels were determined. In general, there was a close association between the reduction in cellular viability and the loss of intracellular enzymes induced by each dust, the chrysotile asbestos samples proving more cytotoxic than the amphiboles. The cytotoxicity of the fibrous dusts was shown to be related to the number of fibers greater than 8 pm in length in the samples.

📜 SIMILAR VOLUMES

Spontaneous and asbestos-induced transfo

Spontaneous and asbestos-induced transformation of mesothelial cells in vitro

✍ I. V. Kravchenko; V. A. Furalyov; L. A. Vasylieva; L. N. Pylev 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 514 KB

The processes of spontaneous and asbestos-induced transformation of rat mesothelium were studied using cell cultures obtained in the laboratory. The same changes in cell properties were established in both spontaneous and asbestos-induced transformation: change in epidermal growth factor (EGF) respo

Cellular immune response in vitro: I. A

Cellular immune response in vitro: I. A requirement for time-dependent T-lymphocyte cell division of cytotoxic cells in the allogeneic response

✍ Robert L. Lundak; Marvin S. Kaplan; Donald J. Raidt; Tina S. Hronis; Gale A. Gra 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 380 KB 👁 1 views

## Abstract Immunocompetent lymphoid cells cultured in vitro with allogeneic stimulator cells have been shown to produce T‐lymphocyte populations which are specifically cytotoxic in vitro to the stimulatory cells whether normal or malignant. Although the culture requirements as well as the allogene

Cyclin D1 and p21 is elevated in the gia

Cyclin D1 and p21 is elevated in the giant cells of giant cell tumors

✍ R. Kandel; S.-Q. Li; R. Bell; J. Wunder; P. Ferguson; A. Kauzman; J.A. Diehl; J. 📂 Article 📅 2006 🏛 Elsevier Science 🌐 English ⚖ 231 KB 👁 1 views

## Abstract Alterations of cell cycle regulatory proteins, especially those that regulate G1 to S transition, have been implicated in the pathogenesis of a wide variety of human tumors. In previous studies we showed that that there is overexpression of cyclin D1 protein predominately in the giant c

1,25-dihydroxyvitamin D3 regulates the e

1,25-dihydroxyvitamin D3 regulates the expression of VDR and NGF gene in Schwann cells in vitro

✍ Anne Cornet; Christel Baudet; Isabelle Neveu; Annick Baron-Van Evercooren; Phili 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 69 KB 👁 2 views

The vitamin D receptor (VDR) is a nuclear receptor that mediates the effect of the active metabolite of vitamin D3, the 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3). To investigate the potential role of this hormone in the peripheral nervous system, we have studied the VDR expression in Schwann cells. Th

The surface structure of murine ascites

The surface structure of murine ascites tumors. II. Difference in cytotoxicity of various phytoagglutinins toward yoshida sarcoma cells in vitro

✍ Motowo Tomita; Tsutoma Kurokawa; Kikuo Onozaki; Toshiaki Osawa; Yoshio Sakurai; 📂 Article 📅 1972 🏛 John Wiley and Sons 🌐 French ⚖ 375 KB

## Abstract Several phytoagglutinins having different sugar specificities were found to exert different agglutinating activity toward murine ascites cell lines. It was also found that some of these phytoagglutinins, especially. Ricinus communis. and. Abrus precatorius. agglutinins, inhibited th

The mutational origin of serum independe

The mutational origin of serum independence in Chinese hamster cells in vitro

✍ Nina B. Varshaver; Marina I. Marshak; N. I. Shapiro 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 French ⚖ 467 KB 👁 1 views

## Abstract The genetic mechanisms determining the ability of transformed cells to grow in a medium with a low serum content (ser^+^) were studied in a clone of Chinese hamster cells with normal serum requirements. The fluctuation test has shwon that serum independence occurs as a random spontaneou