The histone code at DNA breaks: a guide to repair?
β Scribed by van Attikum, Haico; Gasser, Susan M.
- Book ID
- 109958434
- Publisher
- Nature Publishing Group
- Year
- 2005
- Tongue
- English
- Weight
- 499 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1471-0072
- DOI
- 10.1038/nrm1737
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## Abstract ## Background Formation and repair of DNA single-strand breaks are important parameters in the assessment of DNA damage and repair occurring in live cells. The 'Fluorimetric Detection of Alkaline DNA Unwinding (FADU)' method [Birnboim HC, Jevcak JJ. Cancer Res (1981) 41:1889β1892] is a
An episomal DNA vector (YpJA18), encoding two selectable recombinant yeast genes (TRP1, URA3), was constructed to assess the fidelity of DNA repair in haploid repair-competent (RAD) wild-type yeast and several radiation-sensitive mutants. Either a DNA double-strand break (DSB) or a double-strand gap