The fluorescence enhancement of quercetin–nucleic acid system and the analytical application

✍ Scribed by Yuan Liu; Xia Wu; Haiping Zhou; Xiaoyu Liu; Fengju Zhang; Jinghe Yang

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 447 KB
• Volume: 24
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1128

✦ Synopsis

Abstract

Nucleic acid can greatly enhance the fluorescence intensity of quercetin in HMTA‐HCl (pH 5.5) buffer. The enhanced intensity is in proportion to the concentration of nucleic acids in the range 5.0 × 10^−9^ to 1.0 × 10^−6^g/mL for fsDNA, 5.0 × 10^−9^ to 7.0 × 10^−7^g/mL for ctDNA and 5.0 × 10^−9^ to 1.0 × 10^−6^g/mL for yRNA, and their detection limits (S/N = 3) are 3.5 × 10^−9^, 7.8 × 10^−10^ and 2.6 × 10^−9^g/mL, respectively. In comparison with most reported fluorescent probes for the determination of nucleic acids, the proposed probe has higher sensitivity and lower toxicity. The interaction investigation indicates that quercetin binds with double‐strand DNA in groove binding mode, resulting in fluorescence enhancement of this system. Copyright © 2009 John Wiley & Sons, Ltd.