Resonance light-scattering enhancement effect of the protein–Y3+–TTA–SLS system and its analytical application

✍ Scribed by Changying Guo; Xia Wu; Wei Xu; Jinghe Yang

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 203 KB
• Volume: 23
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1053

✦ Synopsis

Abstract

In this paper, a sensitive resonance light scattering (RLS) method for the determination of protein is reported. In the Tris–HCl (pH 7.50) buffer, protein enhanced the RLS intensity of the Y^3+^–2‐thenoyltrifluoroacetone (TTA)–sodium dodecyl sulphate (SLS) system. The enhanced RLS intensities were in proportion to the concentrations of proteins in the range 8.0 × 10^−9^–1.0 × 10^−5^ g/mL for BSA, 1.0 × 10^–8^–1.0 × 10^−5^ g/mL for HSA and 1.0 × 10^–8^–1.0 × 10^−6^g/mL for EA, and their detection limits were 5.0, 5.4 and 6.7 ng/mL, respectively. Actual samples were satisfactorily determined. The interaction mechanism was also studied. Copyright © 2008 John Wiley & Sons, Ltd.