Resonance light scattering study on the interaction of benproperine phosphate with eriochrome blue black R in the presence of sodium dodecylbenzene sulphonate and its analytical application
✍ Scribed by Suling Feng; Jingjing Liu; Xiangming Han; Jing Fan
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 165 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1522-7235
- DOI
- 10.1002/bio.1064
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✦ Synopsis
Abstract
The interaction of benproperine phosphate (BPP) with eriochrome blue black R (EBBR) in the presence of sodium dodecylbenzene sulphonate (SDBS) was studied using resonance light scattering (RLS) technology and ultraviolet‐visual (UV‐vis) spectrophotometry. Under optimum conditions, BPP reacts with EBBP and SDBS to form a three‐component complex, which results in strong RLS signal and a new RLS peak. The enhanced RLS intensities are proportional to the concentration of BPP over the range 0.6–28.0 µg/mL, with a detection limit of 0.053 µg/mL. The affecting factors as well as the influence of coexisting substances were investigated. The results indicate that this assay method could be applied to the determination of BPP in pharmaceuticals, serum and urine samples with satisfactory results. Copyright © 2008 John Wiley & Sons, Ltd.