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The extraction of the enzyme degrading the limonin precursor in citrus albedo

✍ Scribed by Kenneth J. Nicol; Bruce V. Chandler

Publisher: John Wiley and Sons
Year: 1978
Tongue: English
Weight: 553 KB
Volume: 29
Category: Article
ISSN: 0022-5142
DOI: 10.1002/jsfa.2740290909

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Optimum conditions for extracting the enzyme degrading the limonin precursor in citrus albedo involved treatment of the macerated albedo with a commercial pectic enzyme. The release of the enzyme from the cells of the albedo was accompanied by a considerable loss of stability, and the instability of the enzyme once dissociated from the albedo cells, together with its low activity in a highly pectinous source, accounts for the failure of previous attempts at its extraction. A method for proximate assay of enzyme actiyity was developed to monitor the extraction process and to compare the concentrations of enzyme activity in various albedo samples.

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