The effects of kinetin upon epithelium and fibroblasts in tissue culture

✍ Scribed by Mary Faith Orr; Barton McSwain

• Publisher: John Wiley and Sons
• Year: 1957
• Tongue: English
• Weight: 986 KB
• Volume: 10
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(195705/06)10:3<617::aid-cncr2820100329>3.0.co;2-l

✦ Synopsis

SUBSTANCE named kinetin' has been iso-A lated and described as "a physiologically highly active chemical" capable of stimulating cell division. This was demonstrated in tissue cultures o E tobacco "wound" callus tissue. Miller, Skoog, Von Saltza, and Strong2 found that concentrations of kinetin as low as 0.01 parts per million were effective in increasing the cell population of these cultures.

T h e purpose of this communication is to report the results of experiments using kinetin in the nutrient fluid of tissue cultures of adult human skin from the breast and a strain of fibroblasts derived from connective tissue of a carcinoma of the breast.

MATERIALS AKD METHODS Specimens of skin (disease free) were taken from amputated breasts. T h e skin was cut into explants approximately 2 mm. square, removing as much of the dermis as possible, and washed in balanced salt solution. Three explants were embedded, with the dermis side next to the glass, in a clot composed of equal parts chicken plasma and fresh 10-day-oldchick-embryo extract on a no. 1, 12x50 mm., cover glass. These preparations were placed in 16x150 mm. test tubes; 2.5 ml. of nutrient fluid was introduced. T h e tubes were s t o p pered and incubated at 36" C. in a slanted stationary position.

T h e nutrient fluid was composed of 50 per cent Hanks' balanced salt solution, 47 per cent ascitic fluid, and 3 per cent embryo extract with 500 units of penicillin G per cubic centimeter.

Kinetin (5 mg.) was dissolved in distilled water and autoclaved at fifteen pounds of pressure for twenty minutes.