The reactions of fibroblasts in tissue cultures to olive and mineral oils

✍ Scribed by Donley, Dorothy E. ;Hewell, Barbara Ann

• Publisher: Wiley (John Wiley & Sons)
• Year: 1930
• Tongue: English
• Weight: 744 KB
• Volume: 45
• Category: Article
• ISSN: 0002-9106
• DOI: 10.1002/aja.1000450202

✦ Synopsis

There seems to have been a marked tendency, during recent years, to consider the question of the formation of fats in tissue cultures from the pathological rather than the physiological viewpoint. I n Bisceglie and JuhSsz-Schaff e r 's recent publication, "Die Gewebezuchtung in Vitro" ( '28), the only reference, in an extensive bibliography, to intracellular fat deposits is given under the heading "The degenerative processes and death of the culture."

This condition can be explained by the fact that, while the physiological processes connected with fat metabolism in cells may have been the object of much consideration, the questionable accuracy of the microchemical and physical methods which are available for the identification of intracellular fats has tended to discourage more extensive investigation in the field of tissue culture. The need f o r more exact methods of identification of fats and lipoids was particularly emphasized by Kaufmann and Lehmann ('26). The small amount of work done 'in vitro' is in marked contrast to the many reports which, during recent years, have resulted from a study of lipoids in the organism as a whole. This deficit in the general field of tissue culture seemed to warrant a further investigation into the reaction of the cells to various fats. Consequently, a study of the effect of certain vege-Aided by a grant from the Henry Strong Denison Foundation.