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The effects of differential polyadenylation on expression of the dihydrofolate reductase-encoding gene in Chinese hamster lung cells

โœ Scribed by Honghao Yang; Arif Hussain; Peter W. Melera


Book ID
116132947
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
855 KB
Volume
163
Category
Article
ISSN
0378-1119

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Recombinant Chinese hamster ovary (rCHO) cells expressing a high level of chimeric antibody were obtained by cotransfection of heavy-and light-chain cDNA expression vectors into dihydrofolate reductasedeficient CHO cells and subsequent gene amplification in medium containing stepwise increments in m