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Selection strategies for the establishment of recombinant Chinese hamster ovary cell line with dihydrofolate reductase-mediated gene amplification

โœ Scribed by Seung Chul Jun; Min Soo Kim; Jong Youn Baik; Sun Ok Hwang; Gyun Min Lee


Book ID
111706899
Publisher
Springer
Year
2005
Tongue
English
Weight
169 KB
Volume
69
Category
Article
ISSN
1432-0614

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Recombinant Chinese hamster ovary (rCHO) cells expressing a high level of chimeric antibody were obtained by cotransfection of heavy-and light-chain cDNA expression vectors into dihydrofolate reductasedeficient CHO cells and subsequent gene amplification in medium containing stepwise increments in m