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Effects of palindrome structure on dihydrofolate reductase gene amplification in Chinese hamster ovary cells

✍ Scribed by Joon Young Park; Yasuhiro Takagi; Miyuki Yamatani; Kohsuke Honda; Takeshi Omasa; Hisao Ohtake

Book ID: 113682788
Publisher: Elsevier Science
Year: 2009
Tongue: English
Weight: 54 KB
Volume: 108
Category: Article
ISSN: 1389-1723
DOI: 10.1016/j.jbiosc.2009.08.035

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Recombinant Chinese hamster ovary (rCHO) cells expressing a high level of chimeric antibody were obtained by cotransfection of heavy-and light-chain cDNA expression vectors into dihydrofolate reductasedeficient CHO cells and subsequent gene amplification in medium containing stepwise increments in m