The effect of α-amanitin on the synthesis of polyoma specific RNA
✍ Scribed by J.P. Mojardino
- Book ID
- 118312889
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 511 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0006-291X
No coin nor oath required. For personal study only.
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## Abstract Investigations were conducted to test the effects of α‐amanitin on RNA synthesis in preimplantation mouse embryos. Exposure of embryos in culture to 1–100 μg/ml α‐amanitin produced a dose‐ and time‐dependent suppression of total RNA synthesis as measured by incorporation of [^3^H]uridin
## Abstract Actinomycin D and α‐amanitin (an inhibitor of RNA polymerase II) were used to determine the effect of inhibiting RNA synthesis during pre‐implantation mouse embryogenesis. Actinomycin D (0.01 μg/ml) was an effective inhibitor of blastulation and of cleavage from the 1 cell stage on. The