## Abstract Investigations were conducted to test the effects of α‐amanitin on RNA synthesis in preimplantation mouse embryos. Exposure of embryos in culture to 1–100 μg/ml α‐amanitin produced a dose‐ and time‐dependent suppression of total RNA synthesis as measured by incorporation of [^3^H]uridin
The effect of α-amanitin on RNA polymerase II ubiquitination
✍ Scribed by Jozsef Szeberenyi
- Publisher
- The American Society for Biochemistry and Molecular Biology
- Year
- 2006
- Tongue
- English
- Weight
- 504 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1470-8175
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Terms to be familiar with before you start to solve the test: RNA polymerase II, transcription, α‐amanitin, protein ubiquitination, synchronization of cell cultures, affinity adsorption, SDS‐polyacrylamide gel electrophoresis, Western blotting, protein phosphorylation, protein kinases, in vitro transcription, plasmid, promoter, general and regulatory transcription factors, autoradiography.
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