Intracellular ribonuclease from the ciliate Tetrahymena pyriform.is GL was purified 10-fold. After preheating for 20 minutes at 100°C of the ribonuclease preparation 80% of its activity was lost. Preheating under the same conditions, however, in the presence of RNA, did not affect the enzyme activit
The effect of temperature on nucleotide pool formation in Tetrahymena pyriformis
✍ Scribed by Ademar V. de Barros; Jacy F. de Castro; F. T. de Castro
- Publisher
- John Wiley and Sons
- Year
- 1973
- Tongue
- English
- Weight
- 199 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Warming of exponentially growing T. pyriformis to 34°C results in severe inhibition of nucleotide pool formation. The utilization of the pool for stable RNA synthesis is poorly affected at the high temperature. It thus appears that the synthesis and processing of ribosomal RNA precursors are not primarily impaired at 34°C.
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