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A confocal microscopic evaluation of the effects of insulin imprinting on the binding of Concanavalin A by Tetrahymena pyriformis.

✍ Scribed by Peter Kovcs; Christine Sundermann; Barbara H. Estridge; György Csaba

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
484 KB
Volume
19
Category
Article
ISSN
1065-6995

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✦ Synopsis

Abstract

With confocal microscopy it is possible to study the Concanavalin A (Con A) binding characteristics of the surface and interior of a single cell by viewing optical sections. It was observed in Tetrahymena pyriformis that Con A bound both to the plasma membrane and to intracellular structures. Incubation of cells with a competing sugar a‐methylmannopyranoside, decreased binding. Hormonal imprinting with insulin resulted in an increase in binding of Con A to the cell surface and a decrease in intracellular binding. It is possible that the intracellular binding sites may migrate to the plasma membrane.

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