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Effect of temperature on the activity of ribonuclease from Tetrahymena pyriformis

✍ Scribed by Lawrence H. Lazarus; Otto H. Scherbaum


Publisher
John Wiley and Sons
Year
1966
Tongue
English
Weight
217 KB
Volume
68
Category
Article
ISSN
0021-9541

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✦ Synopsis


Intracellular ribonuclease from the ciliate Tetrahymena pyriform.is GL was purified 10-fold. After preheating for 20 minutes at 100°C of the ribonuclease preparation 80% of its activity was lost. Preheating under the same conditions, however, in the presence of RNA, did not affect the enzyme activity. Between 0°C and 41°C the apparent activation energy was 15,600 cal per deg. per mole,


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