The effect of temperature on inhibition of synthesis of cellular macromolecules by Actinomycin D
β Scribed by Y. Z. Ghendon; Olga Blagoveshienskaya; Larisa Babushkina
- Publisher
- Springer Vienna
- Year
- 1971
- Tongue
- English
- Weight
- 162 KB
- Volume
- 35
- Category
- Article
- ISSN
- 1432-8798
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π SIMILAR VOLUMES
Preferential inhibition of the synthesis of ribosomal RNA by low doses of actinomycin D was used to investigate the quantitative relationship between the intensity of silver staining of nucleoli and the rate of RNA-synthesis. The two parameters were found to be strongly correlated in human diploid f
## Abstract A quantitative doseβresponse study was made of the inhibition by actinomycin D of the synthesis of various RNA species in cultured L cells. After a suitable preincubation with various concentrations of actinomycin, synthesis was measured by a brief incorporation of radioactive uridine.
Actinomycin D (0.008 pg/gm of body weight) injected intraperitoneally every two hours, produced a prompt 50% inhibition of RNA synthesis in the jejunum of mice, and a delayed inhibition of DNA synthesis, that reached its maximum inhibition (68% of control values) 4.5 hours after the first injection