## Abstract A quantitative dose‐response study was made of the inhibition by actinomycin D of the synthesis of various RNA species in cultured L cells. After a suitable preincubation with various concentrations of actinomycin, synthesis was measured by a brief incorporation of radioactive uridine.
Correlated inhibition of ribosomal RNA synthesis and silver staining by actinomycin D
✍ Scribed by F. J. Hofgärtner; W. Krone; Kamlesh Jain
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 225 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0340-6717
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✦ Synopsis
Preferential inhibition of the synthesis of ribosomal RNA by low doses of actinomycin D was used to investigate the quantitative relationship between the intensity of silver staining of nucleoli and the rate of RNA-synthesis. The two parameters were found to be strongly correlated in human diploid fibroblasts.
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