✦ LIBER ✦

Delayed inhibition of DNA synthesis in mouse jejunum by low doses of Actinomycin D

✍ Scribed by Renato Baserga; Richard D. Estensen; Robert O. Petersen

Publisher: John Wiley and Sons
Year: 1966
Tongue: English
Weight: 737 KB
Volume: 68
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040680212

No coin nor oath required. For personal study only.

✦ Synopsis

Actinomycin D (0.008 pg/gm of body weight) injected intraperitoneally every two hours, produced a prompt 50% inhibition of RNA synthesis in the jejunum of mice, and a delayed inhibition of DNA synthesis, that reached its maximum inhibition (68% of control values) 4.5 hours after the first injection of actinomycin D. Autoradiographic studies indicated that this low level of actinomycin D inhibited a step in the GI phase of the cell cycle, preventing the initiation, but not affecting the continuation, of DNA biosynthesis. The activity of DNA polymerase was not affected under these conditions. The results are substantially similar to those previously obtained with Ehrlich ascites cells growing in the peritoneal cavity of mice and can be interpreted as indicating that in the GI phase of dividing cells there is a n actinomycin sensitive step whose inhibition prevents the entrance of cells into the DNA-synthesis phase.

Abbreviations DNA, deoxyribonucleic acid RNA, ribonucleic acid dGMP, U M P , dCMP, deoxyribonucleoside 5' monophosphates of guanine, adenine and cytosine, respectively ATP, adenosine 5' triphosphate Tdr, thymidine

📜 SIMILAR VOLUMES

Effect of actinomycin D on protein synth

Effect of actinomycin D on protein synthesis by delayed implanting mouse embryos in vitro

✍ Weitlauf, Harry M. 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 426 KB

## Abstract Blastocysts were collected from the uteri of intact or ovariectomized mice and incubated with or without actinomycin D in a medium containing [^14^C] amino acids. In the absence of actinomycin D the rate of amino acid incorporation by blastocysts from intact mice (i.e., normal embryos)

Inhibition of RNA synthesis by actinomyc

Inhibition of RNA synthesis by actinomycin D: Characteristic dose-response of different RNA species

✍ Robert P. Perry; Dawn E. Kelley 📂 Article 📅 1970 🏛 John Wiley and Sons 🌐 English ⚖ 980 KB

## Abstract A quantitative dose‐response study was made of the inhibition by actinomycin D of the synthesis of various RNA species in cultured L cells. After a suitable preincubation with various concentrations of actinomycin, synthesis was measured by a brief incorporation of radioactive uridine.

Persistent synthesis of 5S RNA when prod

Persistent synthesis of 5S RNA when production of 28S and 18S ribosomal RNA is inhibited by low doses of actinomycin D

✍ Robert P. Perry; Dawn E. Kelley 📂 Article 📅 1968 🏛 John Wiley and Sons 🌐 English ⚖ 861 KB

Cyclic AMP does not mediate inhibition o

Cyclic AMP does not mediate inhibition of DNA synthesis by interferon in mouse Swiss 3T3 cells

✍ Nicolette M. Ebsworth; Joyce Taylor-Papadimitriou; Enrique Rozengurt 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 586 KB

The purpose of this study was to determine whether cyclic AMP (cAMP) plays any direct or indirect role in the antiproliferative effect of mouse L-cell interferon in Swiss 3T3 cells. Firstly, we found that interferon did not affect intracellular levels of cAMP in these cells in the absence or the pre

In vitro DNA synthesis of mouse hepatocy

In vitro DNA synthesis of mouse hepatocytes stimulated by tumor necrosis factor is inhibited by glucocorticoids and prostaglandin D2 but enhanced by retinoic acid

✍ Motonobu Satoh; Masatoshi Yamazaki 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 709 KB

## Abstract We have recently shown that TNF is produced in liver rapidly after partial hepatectomy and that TNF can stimulate DNA synthesis of hepatocyte primary culture with its inhibition by interleukin 6 and transforming growth factor‐β, indicating a pivotal role of TNF and TNF‐driven cytokine i

Reversible inhibition of ribosomal RNA s

Reversible inhibition of ribosomal RNA synthesis in HeLa by lucanthone (Miracil D) with continued synthesis of DNA-like RNA

✍ Robert Bases; Frances Mendez 📂 Article 📅 1969 🏛 John Wiley and Sons 🌐 English ⚖ 824 KB

Ribosomal RNA synthesis was selectively inhibited in HeLa cells by lucanthone, a clinically useful schistosomicide which shares many of the properties of Actinomycin D. Synthesis of DNA-like RNA continued during complete inhibition of ribosomal RNA synthesis. Under these conditions newly synthesized