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The effect of spermidine on endonuclease inhibition by agarose contaminants

✍ Scribed by J.P. Bouché

Book ID
102983478
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
521 KB
Volume
115
Category
Article
ISSN
0003-2697

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✦ Synopsis

Among 14 endonucleases tested, only EcoRl and Hind111 were found to be inhibited by a soluble, ethanol-precipitable material extracted from agarose. This inhibition was raised by a brief preincubation with spermidine (3-5 mM). Inhibition by dextran sulfate of the 14 endonucleases, of DNA ligase, and of the in vitro nick translation was also suppressed by a preincubation with spermidine. We suggest that spermidine acts by forming inactive complexes with polyanions, presumably agaropectins in the case of agarose inhibition.

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