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Inhibition of the activity of restriction endonucleases by spermidine and spermine

✍ Scribed by Malla Kuosmanen; Hannu Pösö


Book ID
115916582
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
374 KB
Volume
179
Category
Article
ISSN
0014-5793

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📜 SIMILAR VOLUMES


The effect of spermidine on endonuclease
✍ J.P. Bouché 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 521 KB

Among 14 endonucleases tested, only EcoRl and Hind111 were found to be inhibited by a soluble, ethanol-precipitable material extracted from agarose. This inhibition was raised by a brief preincubation with spermidine (3-5 mM). Inhibition by dextran sulfate of the 14 endonucleases, of DNA ligase, and