A procedure for the removal of RNA and RNA fragments from large quantities of pBR322 plasmid DNA without the use of RNase is described. Sephacryl S-300 is employed for the separation of low-molecular-weight RNA from plasmid DNA molecules on the basis of gel filtration. The technique thus circumvents
The effect of RNA upon the removal of RNase by phenol treatment
β Scribed by Henry I. Robins; Walter S. McNutt
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 209 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0003-2697
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