When grown on a medium containing 5 g maltodextrin L -1 , Aspergillus niger transformant N402[pAB6-10]B1, which has an additional 20 copies of the glucoamylase (glaA) gene, produced 320 ยฑ 8 mg (mean ยฑ S.E.) glucoamylase (GAM) L -1 in batch culture and 373 ยฑ 9 mg GAM L -1 in maltodextrin-limited chem
The Effect of Organic Nitrogen Sources on Recombinant Glucoamylase Production by Aspergillus niger in Chemostat Culture
โ Scribed by Richard J. Swift; Atul Karandikar; Alison M. Griffen; Peter J. Punt; Cees A.M.J.J. van den Hondel; Geoffrey D. Robson; Anthony P.J. Trinci; Marilyn G. Wiebe
- Book ID
- 115609133
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 105 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1087-1845
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The effects of agitation on fragmentation of a recombinant strain of Aspergillus oryzae and its consequential effects on protein production have been investigated. Constant mass, 5.3-L chemostat cultures at a dilution rate of 0.05 h -1 and a dissolved oxygen level of 75% air saturation, have been co
The response of steady state chemostat cultures of a recombinant Aspergillus niger (B1-D), secreting both a heterologous enzyme (Hen Egg White Lysozyme [HEWL]) and a native enzyme (Glucoamylase), to varying levels of O 2 enrichment of the process gas was evaluated. Formation of both the native and t